This document, created by Pennsylvania State University and the Nanotechnology Applications and Career Knowledge Center (NACK), serves as a guide for students using Veeco ​SPMLab software for processing and analysis of scanning probe microscopy images. The document offers a detailed explanation of items and tools in the Display, Process, and Analysis menues, including images of the menu buttons and their functions. Instructors may provide images and use this document as a lab activity, utilizing the suggested student write-ups included at the end of the guide. In order to access and download this material, users must complete a free registration.